lentiviral vector backbone (Addgene inc)
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Addgene inc
lentiviral vector backbone
Lentiviral Vector Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+backbone+vector/pHR_PGK_antiCD19_synNotch_Gal4VP64+(Plasmid+%2379125)/pm41856998-208-4-8
Average 93 stars, based on 41 article reviews
Lentiviral Vector Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+backbone+vector/pHR_PGK_antiCD19_synNotch_Gal4VP64+(Plasmid+%2379125)/pm41856998-208-4-8
Average 93 stars, based on 41 article reviews
lentiviral vector backbone - by Bioz Stars,
2026-09
93/100 stars
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Synthesized:Article Title: BATTLE: Genetically Engineered Strategies for Split-Tunable Allocation of Multiple Transgenes in the Nervous System Article Snippet: To create the lentivirus-CamKIIa (1.3 kb)-FRT5-iCre-FRT5-WPRE vector, a synthesized transgene FRT5-iCre-FRT5 was inserted into the BamHI-EcoRI site of the lentiviral backbone vector (Addgene 20943). .. Then, a synthesized transgene loxN-FLPOloxN was also inserted into the BamHI-EcoRI site of the Article Title: BATTLE: Genetically Engineered Strategies for Split-Tunable Allocation of Multiple Transgenes in the Nervous System Article Snippet: .. To create the lentivirus-CamKIIa (1.3 kb)-FRT5-iCre-FRT5-WPRE vector, a synthesized transgene FRT5-iCre-FRT5 was inserted into the BamHI-EcoRI site of the Clone Assay:Article Title: Acute deletion of TET enzymes results in aneuploidy in mouse embryonic stem cells through decreased expression of Khdc3 Article Snippet: After 1.5 additional days of growth in SRES without 4-OHT, H2B-EGFP + mESC, resulting from a successful Cre ERT2 -mediated recombination that occurred during 4-OHT treatment, were sorted by FACS (Fluorescence-Activated Cell Sorting) using a FACSAria cell sorter (BD Biosciences). mESC were plated on mitotically inactivated MEFs and allowed to attach for 6 h prior to initiating imaging of the cells for 48 h. Dnmts TKO mESC were previously described , . .. Khdc2 and Khdc3 were cloned into a CMVtight Tet-on advanced Plasmid Preparation:Article Title: Acute deletion of TET enzymes results in aneuploidy in mouse embryonic stem cells through decreased expression of Khdc3 Article Snippet: After 1.5 additional days of growth in SRES without 4-OHT, H2B-EGFP + mESC, resulting from a successful Cre ERT2 -mediated recombination that occurred during 4-OHT treatment, were sorted by FACS (Fluorescence-Activated Cell Sorting) using a FACSAria cell sorter (BD Biosciences). mESC were plated on mitotically inactivated MEFs and allowed to attach for 6 h prior to initiating imaging of the cells for 48 h. Dnmts TKO mESC were previously described , . .. Khdc2 and Khdc3 were cloned into a CMVtight Tet-on advanced Article Title: Baseline cellular state dictates the molecular impact of KRAS mutant variants in pancreatic cancer cells Article Snippet: Whole-exome sequencing libraries were prepared by the Yale Center for Genome Analysis (YCGA) using the IDT xGen V2 Exome Enrichment Kit and sequenced on the Illumina HiSeq platform (paired-end, 100-bp reads) to a mean target coverage of approximately 30×. .. To make KRAS expression constructs (LV-PGK-eGFP-2A-KRAS), other:Article Title: Recording of elapsed time and temporal information about biological events using Cas9. Article Snippet: Theory Recording of elapsed time and temporal information about biological events using Cas9 Expressing:Article Title: Baseline cellular state dictates the molecular impact of KRAS mutant variants in pancreatic cancer cells Article Snippet: Whole-exome sequencing libraries were prepared by the Yale Center for Genome Analysis (YCGA) using the IDT xGen V2 Exome Enrichment Kit and sequenced on the Illumina HiSeq platform (paired-end, 100-bp reads) to a mean target coverage of approximately 30×. .. To make KRAS expression constructs (LV-PGK-eGFP-2A-KRAS), Construct:Article Title: Baseline cellular state dictates the molecular impact of KRAS mutant variants in pancreatic cancer cells Article Snippet: Whole-exome sequencing libraries were prepared by the Yale Center for Genome Analysis (YCGA) using the IDT xGen V2 Exome Enrichment Kit and sequenced on the Illumina HiSeq platform (paired-end, 100-bp reads) to a mean target coverage of approximately 30×. .. To make KRAS expression constructs (LV-PGK-eGFP-2A-KRAS), |
![( A ) Inducible gRNA barcode design: A spacer-blocking hairpin structure is engineered to include a 15-base semirandom genetic barcode within the hairpin loop. In addition, an 8A8G poly(A) tail is appended at the 3′ end of the barcoding gRNA. ( B ) Oligo-CALL system constructs: (i) Dual gRNA barcoding construct: two distinct barcoding gRNA, each driven by human or mouse U6 promoters, separately, with Thy1.1 as a constitutive selection marker. (ii) The green fluorescent protein (GFP) reporter construct: contains three tandem repeats of the barcoding gRNA target site upstream of a miniCMV (minimal cytomegalovirus) promoter controlling GFP expression; blue fluorescent protein (BFP) serves as a selection marker. (iii) CRISPRa construct: constitutively expresses dCas9-VPR [deactivated Cas9 fused to a transcriptional activator VPR (VP64, p65, and Rta)] and the red fluorescent protein (RFP; Cherry variant). All constructs use the <t>lentiviral</t> backbone to facilitate efficient cell transduction. hU6, human U6; mU6, mouse U6; EF1α, elongation factor-1 alpha promoter. ( C ) GFP activation with Oligo-CALL system: In the default state, a self-folding hairpin within the gRNA spacer prevents CRISPRa from activating GFP expression. Introduction of BC-ASOs disrupts this hairpin, exposing the gRNA spacer and enabling robust GFP expression. Representative fluorescence images show strong GFP induction following BC-ASO transfection, in contrast to minimal activation in cells receiving no ASO or mismatched ASOs (mis ASOs). ( D to F ) Flow cytometry analyses of GFP activation in Oligo-CALL–labeled cells: H358 cells bearing BC1 barcodes were transfected with ASOs against BC1. Flow cytometry shows the percentage of GFP + cells (D and E) and the mean fluorescent intensity (F) across the overall population (48 hours post-ASO transfection; n = 2 biological replicates). MFI, mean fluorescence intensity. Cont., control.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4195/pmc12594195/pmc12594195__sciadv.adw9990-f1.jpg)